Selection of bacterial strains when testing antibacterial fabrics
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Antibacterial fabric antibacterial performance test of antibacterial textiles
Species tested included bacteria and fungi. When people evaluate antibacterial properties, the selection of bacterial strains must be scientific and representative. Among bacteria, Gram-positive bacteria (Staphylococcus aureus, Bacillus megaterium, Bacillus subtilis) and Gram-negative bacteria (E. coli, Pseudomonas fluorescens) are mainly used; among fungi, molds (Aspergillus niger, Aspergillus niger, Aspergillus flavus, Aspergillus versicolor, Penicillium citrinum, Trichoderma viride, Chaetomium globosum, Paecilomyces vernieri, Brachyphyllum cereus) and ringworm (Trichophyton gypseum, Trichophyton rubrum, Trichophyton rubrum, Trichophyton violaceum, Trichophyton rubrum) Microsporum, Sporothrix, Candida albicans).
Staphylococcus aureus is a pathogenic bacterium with strong resistance among non-spore bacteria and can be used as a representative of Gram-positive bacteria. Bacillus megaterium is a common pathogenic bacterium among spore-like bacteria; Bacillus subtilis is easy to form spores and has strong resistance, so it can be used as a representative of spore bacteria. Escherichia coli is widely distributed and has been used in various experiments as a representative strain of common Gram-negative bacteria. Aspergillus flavus and Chaetomium globus have been included in the Chinese national standard (GB2423.16-81) as prescribed strains for anti-mold tests. Some other selected molds are common molds that corrode textiles or polymer materials. Candida albicans is a common opportunistic pathogenic fungus in human skin and mucous membranes. It is sensitive to drugs and has the characteristics of a fungus. The colonies resemble bacteria, not bacteria, but are different from mold. Because they have colonies that resemble bacteria, they are easy to count and observe, and are often used as Representative of fungi.
In order to assess whether antibacterial textiles have broad-spectrum antibacterial effects, a more reasonable choice is to mix representative bacterial strains in a certain proportion for testing. For the antibacterial properties of most current antibacterial products, only Staphylococcus aureus, Escherichia coli and Candida albicans are often selected as representatives of Gram-positive bacteria, Gram-negative bacteria and fungi respectively. But in fact, it is far from enough to only use these two bacteria to represent the antibacterial properties of fabrics.
In addition, since most fungi cannot count the number of colonies, the antifungal performance of textiles is mainly evaluated by observing the growth of fungi on the sample after a certain period of time under certain temperature and humidity conditions after the sample is exposed to the fungus. The growth status of fungi is evaluated, and the British standard BS6085-81 is used to evaluate the degree of fungal growth.
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